- Alternate Universe Mode (1080p, 2:23:31): From the Blu-ray: "Watch in Alternate Universe Mode to discover alternate scenes, plot lines, characters and more with the filmmakers as your guide!" This is an entirely different "cut" with a number of scenes presented in early black-and-white storyboard concept stages that build a familiar, but ultimately unique, take on the film compared to its finished product. It sometimes includes humorous "commentary" by Will Gluck.
- Audio Commentary: Co-Writer and Producer Phil Lord, Producer Chris Miller, Directors Bob Persichetti and Peter Ramsey, and Co-Director and Co-Writer Rodney Rothman covers a multitude of filmmaking details.
- "Caught in a Ham" Short (1080p, 4:01): Presented in the style of a vintage WB cartoon, the short film follows Spider-Ham battling the villainous "Dr. Craw-daddy."
- We Are Spider-Man (1080p, 7:51): Cast and crew talk up the benefits of folding multiple Spider-Man variations into the film and discuss some of the core character qualities, Shameik Moore's vocal performance, and more.
- Spider-Verse: A New Dimension (1080p, 5:09): A detailed discussion of the movie's visual style.
- The Ultimate Comics Cast (1080p, 15:02): An in-depth discussion of the film's key voice cast and the qualities the actors brought to the characters.
- Designing Cinematic Comic Book Characters: Heroes & Hams (1080p, 7:45): Filmmakers discuss designing the various Spider characters, both their physical appearances and their personalities.
- Designing Cinematic Comic Book Characters: Scoundrels & Scorpions (1080p, 5:11): Similar to the supplement above but focused on the villains who appear in the film.
- A Tribute to Stan Lee and Steve Ditko (1080p, 8:34): Remembering the icons who first brought Spider-Man to life and passed away within months of one another in 2018.
- The Spider-Verse Super Fan Easter Egg Challenge (1080p, 5:02): The filmmakers share some of the secrets that appear in the film.
- Lyric Videos (1080p): Included are "Sunflower" by Post Malone, Swae Lee (2:47) and "Familia" by Nicki Minaj, Anuel AA, Bantu (3:00).
- Previews (1080p): Additional Sony titles.